TI:Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province
AU:CHENDuan, WANG Huanyu , FU Shihong , SONG Hong , DENG Juan , YANG Yiliang , LIANG Guo2dong1 State
AD:Key Laboratory for Molecular Virology , Fujian Center for Disease Control and Prevention , Fuzhou 350001 ,China Corresponding author : LIANG Guo2dong , E2mail : gdliang @hotmail1com
【Abstract】　Objective 　To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene1 Methods 　PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT2PCR , the PCR products were cloned into T vector for sequencing1 Phylogenetic analysis was carried out by PHYLIP program1 Results 　Newly isolated Japanese encephalitis viruses belonged to genotype Ⅲ, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA1421422 , but had some difference in some domains1 Conclusion 　Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype Ⅲ1 E protein of the isolates showed some differences as compared with vaccine strains1
【Key words】　Encephalitis virus , Japanese ; 　Base sequence ; 　Genotype ; 　Phylogeny