TI:Relationship between core promoter mutation , clinical features and virus replication in HBVcarriers
AU:DAIErhei, LIU Hengjun , SONG Yajun , CHEN Cuiying , YANG Ruifu
AD:The Fifth Hospital of Shijiazhuang ,Shijiazhuang 050021 ,China
【Abstract】　Objective 　To elucidate the relationship between HBV core promoter mutation and clinical features as well as its effects on serum e system and viral replication1 Methods 　Semi2nested mutation specific PCR (msPCR) was employed for detecting core promoter mutation at nt 1 76221 764 in 97 patients with HBV infection1 Results 　The msPCR method was demonstrated to be specific and reliable for the mutation detection by sequencing the PCR products1 The detection ratio of the mutation in patients with acute hepatitis , mild , moderate and severe chronic hepatitis and liver cirrhosis was 2P5 , 7P43 , 10P31 , 1P3 and 7P15 , respectively1 The detection rate of the mutation in liver cirrhosis was significantly higher than that in light chronic hepatitis ( P < 01025) 1 In 92 patients with chronic HBV infection , HBeAg positive rate in wild (25P92) , mutant (42P92) and mixed (25P92) strain infection was 8010 % , 5610 % and 6413 % , HBV DNA level was (414 ±815) ×108 , (111 ±116) ×109 and (114 ±118) ×109 copiesPml , the rate of abnormal ALT was 4410 % , 5210 % and 4216 %;ALT level was (5816 ±7910) , (5711 ±7512) and (6216 ±9013) IUPL , respectively ( P > 0105) 1 Conclusions 　The msPCR method for detecting core promoter mutation at nt 1 76221 764 is specific and reliable1 Core promoter mutation is associated with the severity of liver disease , but neither related to the status of e system in serum nor to the virus replication1
【Key words】　Hepatitis B virus ; 　Mutation ; 　Polymerase chain reaction