TI:Detection of multiresistance Aeromonas with TEM type resistant genes in patients with cirrhosis
AU:QU Fen ,BAO Chunmei , CUI Enbo , SHI Jiabin , GUO Tongsheng ,MAO Yuanli
AD:The Center of Clinical Laboratory , The No. 302 Hospital of The People’s Liberation Army , Beijing 100039 ,China
【Abstract】　Objective 　To study the status ofβ2lactamase produced by multiresistant Aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading1 Methods Four multiresistant Aeromonas strains isolated from serious liver cirrhosis patients from the No1302 hospital1 The TEM resistant genes were detected by PCR and agarose gel electrophoresis1 Results 　Three TEM21 positive strains were detected from four multiresistant Aeromonas isolates consisting of one Aeromonas sobria and three Aeromonas hydrophila isolated from blood and ascites1This was further confirmed by gene sequencing1The multiresistance to antibiotics was higher in four Aeromonas isolates1All strains tested were resistant to ampicillin , cefazolin and cefmetazole1The cirrhosis patients who suffered from Aeromonas infection had poor prognosis and had mortality rate of 3P41 Conclusion 　Theβ2lactamase TEM21 resistant genes was detected in clinical multiresistant Aeromonas strain isolated from serious cirrhosis patients1The results suggested that TEM21 was the main resistance mechanism of Aeromonas strain and was reduced by adding enzyme inhibitor1
【Key words】　Liver cirrhosis ; 　Aeromonas ; 　Resistant gene