TI:Expression of HBV preS2PS gene in mammalian cells transferred with adenoviral vector
AU:LEI Chunliang ,HUANG Chenghui , YANG Zhan , TANG Xiaoping
AD:The Eighth Hospital of Guangzhou , Guangzhou 510060 ,China
【Abstract】　Objective 　To study HBV preS2 PS gene expression effects in mammalian cells transferred with recombinant adenoviral vector1 Methods 　The replication2deficient recombinant adenoviral vector (Ad2HBs) carrying HBV preS2 PS gene were constructed by homologous recombination in bacteria1 The 293 cells , Vero cells ,HepG2 cells and mesenchymal stem cells (MSCs) were infected with adenoviruses1 The expressions of enhanced green fluorescent protein (EGFP) were observed with fluorescence microscope and the expressions of HBsAg were detected by RT2PCR and ELISA in vitro1 Results 　More than 90 % of 293 cells , Vero cells , HepG2 cells or MSCs expressed EGFP after transfection at the MOI of 20 and the titers of HBsAg were more than 31229 (A value) in culture supernatant1 Conclusion 　The HBV preS2 PS gene was not only expressed efficiently in immortalized cells , but also expressed efficiently in stem cells with the recombinant adenoviruses vector1
【Key words】　Adenoviridae infections ; 　Hepatitis B virus ; 　Stem cells