TI:Identification of oligopeptides mimicking the virus attachment protein of hantaan virus
AU:LB Xin , XUEXiaoping , YANG Qiaoxin , YIN Wen , LEI Yingfeng , ZHANG Fanglin
AD:Department of Microbiology , The Fourth Military Medical University , Xi’an 710032 , China
【Abstract】　Objective 　To identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus1 Methods 　The monoclonal antibody 3G1 was used as the ligand to biopan from a phage2 displayed 122amino acid peptide library , then the positive phage clones were chosen and sequenced1 The amino acid sequences of them were compared with that of hantaan virus G2 in homology1 The characteristics of positive phage were studied by IFA and ELISA1 A decapeptide combinding to cell membrane was observed under laser scannning confocal microscope (LSCM) 1 Results 　The conservative motif PX(122) HX(022) H displaying on positive clones shared homologous amino acid sequence with G2 96YPWHTAKCHY105 1 Conclusion 　G2 96YPWHTAKCHY105 might play some roles in virus binding to host cell , and might be a possible key epitope of hantaan virus VAP1
【Key words】　Hantaan virus ; 　Glycoproteins ; 　Epitope