TI:Inoculation of plasmid expressing the dengue 2 NS1 gene elicits immunity in mice　
AU:YANG Chunyu , CHENYuan , YAN Huijun , FANG Danyun , XUE Yaohua , JIANGLifang
AD:Department of Microbiology , Sun Yat2sen University of Medical Sciences , Guangzhou 510089 , China Corresponding author : YANG Chun2yu , Tel : 862041624258618 , E2mail : ychunyucn @yahoo1com1cn
【Abstract】　Objective 　To study cellular and humoral immune responses to NS1 protein in mice inoculated intramuscularly with recombinant plasmid expressing dengue 2 NS1 gene1 Methods 　The eukaryotic expressing plasmid pCNX22NS1 was injected into tibialis anterior muscle in mice1 The mice were subsequently boosted with the same dose and same method twice after the initial inoculation1 The mice were killed at four2week intervals and their serum and spleen cells were harvested for further test1 Results 　Dengue 2 antibodies were detectable in the sera from inoculated animals four weeks after the last boost1 The changes of CD4 + T lymphocyte and CD8 + T lymphocyte were also determined by flow cytometry1 Conclusion 　The recombinant plasmid containing dengue 2 NS1 genes is immunogenic in intramuscularly inoculated mice1 The vaccinated mice produced dengue22 specific and long lasting immunity1
【Key words】　Dengue virus ; 　Genes , NS1 ; 　Vaccines , DNA