TI:Establishment and application of fluorescent quantitative PCR method for detecting human herpes virus type 6
AU:DONG Xuejun, SHI Changgen , QU Shiwen , LIU Zhongmin , FU Jiaping
AD:Central Laboratory of Shaoxing People’s Hospital , Shaoxing 312000 ,China
【Abstract】　Objective 　To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6) 1 Methods 　According to the specific sequence of human herpes virus type 6 genes , the primers and the fluorescent probe(TaqMan) were designed and synthesized1 The fragment generated from HHV 6 gene as template was cloned into the pMD182T vector which was constructed from the pUC 181 And the positive recombinant plasmid was 1∶10 diluted and used as standard quantitative template to make the standard curve for sample detection1 Results 　The standard curve indicated the linear relationship between Ct (cycle threshold) and template concentration1 The clinical samples from 135 cases were detected by this system ,16 cases among 135 were positive1 Conclusion 　The fluorescent quantitative PCR method for the detection of human herpes virus type 6 is simple and accurate , and this method may be helpful to clinical diagnosis1
【Key words】　Herpesvirus 6 ,human ; 　TaqMan probes ; 　Polymerase chain reaction