TI:Effect of Chinese herbal medicine Xin2kang oral liquid on interferon2induction and its antiviral activity in Coxsackievirus B3 infected mice 　
AU:WAN Sujun, LI Jiannong , ZHAO Hong , WANG Lixia , HUANG Xiazhen, ZHU Yan , CHEN Hongshan
AD:Guang An Men Hospital , The Chinese Academy of Traditional Chinese Medicine , Beijing 100053 , China
【Abstract】　Objective 　To investigate the effect of Chinese herbal medicine Xin2kang oral liquid on interferon ( IFN)2induction and its antiviral activity in Coxsackievirus B3 myocardiotropic virus strain (CVB3) infected mice1 Methods 　The Xin2kang oral liquid was given orally to mice two days prior to the challenge of CVB3 virus to induce myocarditis1 Two dosages of Xin2kang oral liquid crude herbal medicine 30 g·kg 21·d 21 and 12 g·kg 21·d21 were given to the mice of different treatment groups respectively , sterilized water was given to the mice of virus control group1 IFN2α106 U·kg 21·d21 S1C was given to the infected mice as positive drug control group1The mice were sacrificed on 5th , 10th and 20th day of infection for evaluation , the levels of serum interferon ( IFN) were titrated with vesicular stomatitis virus (VSV) and cardiac tissue was fixed and sectioned1 The quantitative histological changes at various stages of myocarditis were observed1 Results 　In the infected mice fed with 30 g· kg21·d21 or 12 g·kg21·d21 of Xin2kang oral liquid orally for 5 ,10 and 20 days , the mean titer of serum IFN of Xin2 Kang oral liquid treated group was markedly higher (2913 UP011 ml) than that of virus control group (1216 UP011ml) 1 The level of serum IFN in IFN treated positive control mice was lower than that of Xin2kang treatment groups1The histological examination showed extensive myocardial necrosis and cellular infiltration in virus control group , but necrosis and cellular infiltration were less severe in Xin2kang treatment groups of mice1 It is demonstrated that there were close correlation between the degree of myocardial lesions and the level of IFN2induction in treated mice1 Conclusion 　Xin2kang oral liquid could facilitate the induction of endogenous interferon that exerted its antiviral activity in CVB3 infected mice1 This can help us to understand better the mechanism of anti2 CVB3 effect of Xin2Kang oral liquid1
【Key words】　Drugs ,Chinese herbal ; 　Coxsackievirus infections ; 　Interferons ; 　Myocardium