TI:Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses
AU:CHEN JimingWANG Zhiliang , PANG Zengchang , SUN Chengying , SUN Yingxue , SONG Cuiping , YI Ying
AD:National Diagnostic Center of Animal Exotic Infectious Diseases , Animal Quarantine Institute of the Ministry of Agriculture , Qingdao , 266032 , China Corresponding author :WANG Zhi2liang , E2mail : zlwang @public1 qd1sd1cn , Tel :053227839922
【Abstract】　Objective 　To design and rapidly evaluate a TaqMan assay for detecting influenza A viruses1 Methods 　The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5101 Their specificity and conservation were verified through Blast in GenBank and electronic hybridization1 The assay′s sensitivity was compared with the standard RT2PCR1 Results 　The designed primers and probe were confirmed to be very specific and conserved1 The assay was 3227 folds more sensitive than the standard RT2PCR1 The RT and PCR steps could be simplified into one step1 Conclusion 　The TaqMan Real2time PCR assay is specific , sensitive and easy to perform1
【Key words】　Orthomyxoviridae ; 　Polymerase chain reaction