TI:Antigen Analysis of Angiost rongylus cantonensis in Different Developmental Stages

AU:L I Hua, CHEN Xiaoguang, SHEN Haoxian , PENG Hongjuan , ZHAO Xingcun

AD:( Depa rtment of Pa rasitology , Southern Medical University , Guangzhou 510515 , China)

【Abstract】　Objective 　To analyze the difference among antigens of A ngiost rongylus cantonensis in different develop2 mental stages and identify dominant diagnostic antigen for angiostrongyliasis. 　Methods 　Antigens of A . cantonensis in differ2 ent developmental stages were analyzed by SDS2PAGE and immunoblot . 　Results 　The protein bands of all developmental stages were similar on SDS2PAGE. The Mr 40 000 , 50 000 , 66 000 and 80 000 antigens reacted not only with the sera of rats infected by A . cantonensis but also with the sera of normal rats. The Mr 104 000 antigen could be discerned by sera of rats in2 fected with A . cantonensis for 2 weeks. The Mr 32 000 antigen could be recognized by sera of rats 2 weeks after infection , and the reaction became stronger with the infection continued. 　Conclusion 　The Mr 40 000 , 50 000 , 66 000 and 80 000 anti2 gens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A . cantonen2 sis . The Mr 104 000 of larva , Mr 33 000 of adult females and Mr 32 000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis.

【Key words】　Angiostrongylus cantonensis ; Antigen ; Immunodiagnosis Supported by the Key Technologies R &D Programmes of the Ministry of Science and Technology(2003BA712A03207)